ChIP-on-chip
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- ChIP-on-chip search for term
Chromatin immunoprecipitation (ChIP) combined with microarray detection. Usually, cells are treated with a crosslinking reagent (for example, formaldehyde), which is used to covalently link protein complexes in situ to DNA. The crosslinked chromatin is then isolated and fragmented. An immunoprecipitation step is used to enrich the protein of interest together with crosslinked DNA fragments. To identify and quantify these DNA fragments, the crosslinks are reversed and the DNA fragments are usually labelled with a fluorescent dye and hybridized to microarrays with probes that correspond to genomic regions of interest. (Fan 2006)
