Whole-genome shotgun (WGS) strategy


Whole-genome shotgun (WGS) strategysearch for term

involves the assembly of sequence reads generated in a random\, genome-wide fashion. In this approach\, the entire genome of the target genome (chromosome) is fragmented into pieces of defined sizes that are subcloned into plasmid vectors or directly sequenced via NGS technologies. Sequence reads are generated from many subclones so as to produce highly redundant sequence coverage across the genome or chromosome. The sequences are then assembled using various computational methods to produce a consensus sequence. (Feuillet 2011)