De novo transcriptome assembly
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- De novo transcriptome assembly search for term
The de novo transcriptome assembly strategy does not use a reference genome: it leverages the redundancy of short-read sequencing to find overlaps between the reads and assembles them into transcripts. A handful of de novo transcriptome assemblers have been developed. The Rnnotator, Multiple k and Trans-ABySS assemblers follow the same strategy: they assemble the data set multiple times using a De Bruijn graph-based approach to reconstruct transcripts from a broad range of expression levels and then post-process the assembly to merge contigs and remove redundancy. By contrast, other assemblers (such as Trinity and Oases) directly traverse the De Bruijn graph to assemble each isoform. (Martin 2011)
